The T-47 line was isolated by I. Keydar from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast. T-47D is a human breast cancer cell line commonly used in biomedical research involving the hormonal expression of cancer cells. Characteristics. The cells were derived from the pleural effusion of a ductal carcinoma found in the mammary gland of an elderly human patient.
of NCI-PBCF- HTB133 (T-47D) Vial Start-up The cryoprotectant (DMSO) should be removed by centrifugation. The seeding density to use with a vial of T-47D cells is 1 vial into one T-25 flask with10 mL medium or about 1 x 10. 5. viable cells/cm. 2. Complete growth medium The complete growth medium used to expand T-47D cells is RPMI 1640 + 10%. Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease.
May 06, 2017 · The T47D cell line is an ideal experimental model to elucidate the progesterone-specific effects of a luminal A subtype of breast cancer. Yu S(1), Kim T(2), Yoo KH(3), Kang K(4). Author information: (1)Department of Clinical Laboratory Science, Cited by: 8. T-47D xenograft model. Breast cancer is responsible for over 40,000 deaths annually in the United States, as per the American Cancer Society (ACS). Xenograft studies are based on direct implantation of tumor cell lines into various organs of immunodeficient mice.